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Image Search Results
Journal: bioRxiv
Article Title: Toward Improving Immunotolerance for Stem Cell-Derived Islets
doi: 10.1101/2022.09.15.508091
Figure Lengend Snippet: Reconstitution of the immune tolerant environment using cytokine cocktails. A. Cytokine array of secreted cytokines and chemokines in testis supernatant. The spots on the top left, top right and bottom left were the reference spots. B. Quantification of cytokines and chemokines normalized to the blank medium control. C. IL-2 and IL-10 Elisa of testis supernatant confirm secretion of cytokines in the testis. D. Cytokines screening for induction of T regs in by CD3/CD28 beads. 3 days after expansion, MNCs were collected for CD25 and CD4 staining. E. T cell stimulated by CD3/CD28 beads while adding IL-2, IL-10, TGFβ, or a combination of cytokines, measured 3 days after co-culture. Upper row, T regs induction under each condition. Lower row, T cell proliferation tested by CFSE cell proliferation trace under each condition. F. Immunostaining of IL-2, INS, FOXP3, and CD8 in grafts at 10 days in the control group, and 2, 4, and 8 weeks show a protective effect of the cytokine cocktails (bar = 100 μm).
Article Snippet: 48 hours after culturing, the testis supernatant and the blank medium were collected for the
Techniques: Control, Enzyme-linked Immunosorbent Assay, Staining, Co-Culture Assay, Immunostaining